Single Molecule Discussion Group

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About the Single Molecule Discussion Group

The SMDG series started in September 2006 and are organized by Tamara Chiba. This is a great place where faculty, postdocs and students meet once a month to discuss single molecule research conducted at Yale as well as litterature in a relaxed and informal atmosphere with great food from Nica's Market!

The SMDG meetings are unique in bringing together researchers from various departments, including Physics, Engineering and Applied Sciences, Molecular Biophysics and Biochemistry, and Biology to discuss topics of common interest.

During the first 30 min a speaker presents his/her current research work as well as the various difficulties they might have encountered. This is an excellent opportunity to discuss and exchange ideas amongst the diverse group. The second half of the meeting is dedicated to the presentation and discussion of a journal article by a second speaker.

The speakers are comprised of faculty, postdocs, and students. We encourage volunteers to come forward for a presentation. Remember it's only a 30 min discussion, and you don't have to have cool data yet, just talk about what you are working on.

Hope to see you there!

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List of Sponsors

We greatly thank our sponsors:

2006/2007: MikroMasch, Veeco, Anaspec, Novascan, Olympus, Chroma, Coherent, Newport

2007/2008: Photon Technology International, NanoAndMore, TedPella, Yale Dean's Fund

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Meeting schedule

12-1 pm Bass 205

Food available from Nica's market at 11:45

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Spring 2008

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Friday, February 8

Research: Andy Schaefer from the lab of Paul Forscher

Paper: Andrew Mack from the lab of Simon Mochrie "DNA Translocation and Loop Formation Mechanism of Chromatin Remodeling by SWI/SNF and RSC" Zhang et al.

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February 29th

Special Leap Day Seminar

Matthew Lang

Biological Engineering, MIT

“Advances in Single Molecule Biophysics: Measurements of Kinesin and Actin Machinery”

Friday Feb 29th, Bass 405 12-1pm, Food from Nica's Market at 11:45


Abstract: An overview of a number of single molecule biophysics advances will be provided including combined optical trapping and single molecule FRET, new strategies for forming single molecule assays using the M13 bacteriophage as a linker, and demonstration of trapping objects on silicon wafers. Measurements will also be presented that test a novel mechanism for the kinesin power stroke, recently proposed by Hwang, Lang and Karplus. The kinesin measurements compare stall forces and single molecule motility records for wild type vs. mutants in the cover neck bundle. The development of an assay for probing rupture force between single actin filaments and actin binding proteins will also be presented. These studies compare the likelihood of unfolding vs. unbinding in filamin and a-actinin.

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March 28th

Research: Kristina Herbert from the lab of Joan Steitz

Paper: Brannon McCullough from the lab of Enriqe De La Cruz

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April 18th

Research: Aditya Paul from the lab of Thomas Pollard

Paper: Volunteer?

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May 16th

Research: Michael Bruno from the lab of Anna Pyle

Paper: Chad McCormick from the lab of Thomas Pollard

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Fall 2007

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Friday, December 7th

Research: Cecile Mejan from the lab of Eric Dufresne "Probing Mechanotransduction Using Close Loop Force Measurements"

Paper: Margaret Trias from the lab of Simon Mochrie Analysis of Single-Molecule FRET Trajectories Using Hidden Markov Modeling

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Friday, November 2nd

Research: Liz Middleton from the lab of Liz Rhoades "Using Single Molecule and Fluorescence Methods to Study Membrane Interactions of alpha-Synuclein"

Paper: Kendra Frederick from the lab of Enrique De La Cruz, Myosin V Walks by Lever Action and Brownian Motion

Sponsored by TedPella

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Friday October 5th

special discussion: what is an intermediate?

Paper: Doro Blaho from the lab of Andrew Miranker, Dissecting the multistep reaction pathway of an RNA enzyme by single-molecule kinetic ‘‘fingerprinting’’

sponsored by NanoAndMore

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Friday, September 21st

Research: Gregg Lois from the lab of Corey O'Hern "Physical Models of Protein Folding"

Paper: Lynne Regan, Direct Measurement of the Full, Sequence-Dependent Folding Landscape of a Nucleic Acid

sponsored by Photon Technology International

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